Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/12: Difference between revisions

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(Autocreate 2014/05/12 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
 
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==mm/dd/yyyy==
==mm/dd/yyyy==
* Insert content here...
Ran PCR products from 5/6/14 on a gel. Only 6, RhlI worked. <br><br>
Re-ran other PCRs, annealing temp 40, 4min ext
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Number'''
| align="center" style="background:#f0f0f0;"|'''Template'''
| align="center" style="background:#f0f0f0;"|'''F Primer'''
| align="center" style="background:#f0f0f0;"|'''R Primer'''
| align="center" style="background:#f0f0f0;"|'''Annealing T'''
| align="center" style="background:#f0f0f0;"|'''Expected length'''
|-
| 1||pTet-mCh 2/14/14 74ng||P127||P097||53||3148
|-
| 2||LuxI sender const 8/31/13 179ng||P063||P064||23||654
|-
| 3||K084007 3/10/13 4-2-1||P069||P070||32||645
|-
| 4||K084007 3/10/13 4-2-2||P069||P070||32||645
|-
| 5||RBS-LasI 3/8/13 31ng||P069||P070||32||645
|}
<br>
Ran a second one of the backbone with annealing temp 50, ext time 4 min
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Label'''
| align="center" style="background:#f0f0f0;"|'''Template'''
| align="center" style="background:#f0f0f0;"|'''F Primer'''
| align="center" style="background:#f0f0f0;"|'''R Primer'''
| align="center" style="background:#f0f0f0;"|'''Annealing T'''
| align="center" style="background:#f0f0f0;"|'''Expected length'''
|-
| mCh sender||pTet-mCh 2/14/14 74ng||P127||P097||53||3148
|}
<br><br>
Ran on gel, only 5, LasI worked.
<br><br>
Ran a third, annealing temp 30
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Label'''
| align="center" style="background:#f0f0f0;"|'''Template'''
| align="center" style="background:#f0f0f0;"|'''F Primer'''
| align="center" style="background:#f0f0f0;"|'''R Primer'''
| align="center" style="background:#f0f0f0;"|'''Annealing T'''
| align="center" style="background:#f0f0f0;"|'''Expected length'''
|-
| LuxI||LuxI sender const 8/31/13 179ng||P063||P064||23||654
|}




Revision as of 16:07, 12 May 2014

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mm/dd/yyyy

Ran PCR products from 5/6/14 on a gel. Only 6, RhlI worked.

Re-ran other PCRs, annealing temp 40, 4min ext

Number Template F Primer R Primer Annealing T Expected length
1 pTet-mCh 2/14/14 74ng P127 P097 53 3148
2 LuxI sender const 8/31/13 179ng P063 P064 23 654
3 K084007 3/10/13 4-2-1 P069 P070 32 645
4 K084007 3/10/13 4-2-2 P069 P070 32 645
5 RBS-LasI 3/8/13 31ng P069 P070 32 645


Ran a second one of the backbone with annealing temp 50, ext time 4 min

Label Template F Primer R Primer Annealing T Expected length
mCh sender pTet-mCh 2/14/14 74ng P127 P097 53 3148



Ran on gel, only 5, LasI worked.

Ran a third, annealing temp 30

Label Template F Primer R Primer Annealing T Expected length
LuxI LuxI sender const 8/31/13 179ng P063 P064 23 654



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