Difference between revisions of "Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/06"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
 
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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==mm/dd/yyyy==
 
==mm/dd/yyyy==
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Ryan set up PCR. Annealing temp 53, extension time 3:30min. Diluted each template down 1:1000.
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{| {{table}}
 
{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Number'''
 
| align="center" style="background:#f0f0f0;"|'''Template'''
 
| align="center" style="background:#f0f0f0;"|'''Template'''
 
| align="center" style="background:#f0f0f0;"|'''F Primer'''
 
| align="center" style="background:#f0f0f0;"|'''F Primer'''
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| align="center" style="background:#f0f0f0;"|'''Expected length'''
 
| align="center" style="background:#f0f0f0;"|'''Expected length'''
 
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| mCherry sender intermediate vector||P127||P097||53||3148
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| 1||pTet-mCh 2/14/14 74ng||P127||P097||53||3148
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| 2||LuxI sender const 8/31/13 179ng||P063||P064||23||654
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| 3||K084007 3/10/13 4-2-1||P069||P070||32||645
 
|-
 
|-
| LuxI||P063||P064||23||
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| 4||K084007 3/10/13 4-2-2||P069||P070||32||645
 
|-
 
|-
| LasI||P069||P070||32||
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| 5||RBS-LasI 3/8/13 31ng||P069||P070||32||645
 
|-
 
|-
| RhlI||P075||P076||39||
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| 6||RhlI sender const 9/27/13 72 ng||P075||P076||39||642
 
|}
 
|}
 
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<br>
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<br>
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Ryan ran gel of colony PCRs from last week.
  
 
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Latest revision as of 23:56, 26 September 2017

Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

mm/dd/yyyy

Ryan set up PCR. Annealing temp 53, extension time 3:30min. Diluted each template down 1:1000.

Number Template F Primer R Primer Annealing T Expected length
1 pTet-mCh 2/14/14 74ng P127 P097 53 3148
2 LuxI sender const 8/31/13 179ng P063 P064 23 654
3 K084007 3/10/13 4-2-1 P069 P070 32 645
4 K084007 3/10/13 4-2-2 P069 P070 32 645
5 RBS-LasI 3/8/13 31ng P069 P070 32 645
6 RhlI sender const 9/27/13 72 ng P075 P076 39 642



Ryan ran gel of colony PCRs from last week.