Difference between revisions of "Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/04/28"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
 
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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{| {{table}}
 
{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Number'''
 
| align="center" style="background:#f0f0f0;"|'''Template'''
 
| align="center" style="background:#f0f0f0;"|'''Template'''
 
| align="center" style="background:#f0f0f0;"|'''F primer'''
 
| align="center" style="background:#f0f0f0;"|'''F primer'''
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| align="center" style="background:#f0f0f0;"|'''Product'''
 
| align="center" style="background:#f0f0f0;"|'''Product'''
 
|-
 
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| Receiver int||P123||P112||49||2203||2430||Rec backbone
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| 1||Receiver int||P123||P112||49||2203||2430||Rec backbone
 
|-
 
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| Receiver int||P119||P107||48||1051||||pLux
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|2|| Receiver int||P119||P107||48||1051||||pLux
 
|-
 
|-
| Receiver int||P125||P107||48||1051||||pRhl
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| 3||Receiver int||P125||P107||48||1051||||pRhl
 
|-
 
|-
| Receiver int||P126||P107||48||1051||||pEsa
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| 4||Receiver int||P126||P107||48||1051||||pEsa
 
|-
 
|-
| F2620||P113||P114||51||817||||LuxR
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|5|| F2620||P113||P114||51||817||||LuxR
 
|-
 
|-
| K092900+eCFP (70ul/ng)||P117||P118||67||765||||RhlR
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| 6||K092900+eCFP (70ul/ng)||P117||P118||67||765||||RhlR
 
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|-
| K092900+eCFP (74ng/ul)||P117||P118||67||765||||RhlR
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| 7||K092900+eCFP (74ng/ul)||P117||P118||67||765||||RhlR
 
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| K092900 (96ng/ul)||P117||P118||67||765||||RhlR
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|8|| K092900 (96ng/ul)||P117||P118||67||765||||RhlR
 
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|-
| EsaR synthesized||P121||P122||50||758||||EsaR
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| 9||EsaR synthesized||P121||P122||50||758||||EsaR
 
|}
 
|}
  

Latest revision as of 22:56, 26 September 2017

Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

mm/dd/yyyy

PCR for Receiver assemblies. Diluted all templates 1:1000 in water, annealing temp 48, extension time 2:20

Number Template F primer R primer Tm Expected length Expected incorrect length Product
1 Receiver int P123 P112 49 2203 2430 Rec backbone
2 Receiver int P119 P107 48 1051 pLux
3 Receiver int P125 P107 48 1051 pRhl
4 Receiver int P126 P107 48 1051 pEsa
5 F2620 P113 P114 51 817 LuxR
6 K092900+eCFP (70ul/ng) P117 P118 67 765 RhlR
7 K092900+eCFP (74ng/ul) P117 P118 67 765 RhlR
8 K092900 (96ng/ul) P117 P118 67 765 RhlR
9 EsaR synthesized P121 P122 50 758 EsaR