Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/04/28: Difference between revisions

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==mm/dd/yyyy==
==mm/dd/yyyy==
PCR for Receiver assemblies
PCR for Receiver assemblies. Diluted all templates 1:1000 in water, annealing temp 48, extension time 2:20


{| {{table}}
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Number'''
| align="center" style="background:#f0f0f0;"|'''Template'''
| align="center" style="background:#f0f0f0;"|'''Template'''
| align="center" style="background:#f0f0f0;"|'''F primer'''
| align="center" style="background:#f0f0f0;"|'''F primer'''
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| align="center" style="background:#f0f0f0;"|'''Product'''
| align="center" style="background:#f0f0f0;"|'''Product'''
|-
|-
| Receiver int||P123||P112||49||2203||2430||Rec backbone
| 1||Receiver int||P123||P112||49||2203||2430||Rec backbone
|-
|-
| Receiver int||P119||P107||48||1051||||pLux
|2|| Receiver int||P119||P107||48||1051||||pLux
|-
|-
| Receiver int||P125||P107||48||1051||||pRhl
| 3||Receiver int||P125||P107||48||1051||||pRhl
|-
|-
| Receiver int||P126||P107||48||1051||||pEsa
| 4||Receiver int||P126||P107||48||1051||||pEsa
|-
|-
| F2620||P113||P114||51||817||||LuxR
|5|| F2620||P113||P114||51||817||||LuxR
|-
|-
| K092900+eCFP||P117||P118||67||765||||RhlR
| 6||K092900+eCFP (70ul/ng)||P117||P118||67||765||||RhlR
|-
|-
| EsaR synthesized||P121||P122||50||758||||EsaR
| 7||K092900+eCFP (74ng/ul)||P117||P118||67||765||||RhlR
|-
|8|| K092900 (96ng/ul)||P117||P118||67||765||||RhlR
|-
| 9||EsaR synthesized||P121||P122||50||758||||EsaR
|}
|}


Revision as of 12:49, 29 April 2014

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mm/dd/yyyy

PCR for Receiver assemblies. Diluted all templates 1:1000 in water, annealing temp 48, extension time 2:20

Number Template F primer R primer Tm Expected length Expected incorrect length Product
1 Receiver int P123 P112 49 2203 2430 Rec backbone
2 Receiver int P119 P107 48 1051 pLux
3 Receiver int P125 P107 48 1051 pRhl
4 Receiver int P126 P107 48 1051 pEsa
5 F2620 P113 P114 51 817 LuxR
6 K092900+eCFP (70ul/ng) P117 P118 67 765 RhlR
7 K092900+eCFP (74ng/ul) P117 P118 67 765 RhlR
8 K092900 (96ng/ul) P117 P118 67 765 RhlR
9 EsaR synthesized P121 P122 50 758 EsaR


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