Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/04/28

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PCR for Receiver assemblies. Diluted all templates 1:1000 in water, annealing temp 48, extension time 2:20

Number Template F primer R primer Tm Expected length Expected incorrect length Product
1 Receiver int P123 P112 49 2203 2430 Rec backbone
2 Receiver int P119 P107 48 1051 pLux
3 Receiver int P125 P107 48 1051 pRhl
4 Receiver int P126 P107 48 1051 pEsa
5 F2620 P113 P114 51 817 LuxR
6 K092900+eCFP (70ul/ng) P117 P118 67 765 RhlR
7 K092900+eCFP (74ng/ul) P117 P118 67 765 RhlR
8 K092900 (96ng/ul) P117 P118 67 765 RhlR
9 EsaR synthesized P121 P122 50 758 EsaR