Difference between revisions of "Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/04/28"

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(Autocreate 2014/04/28 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
 
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PCR for Receiver assemblies
  
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Template'''
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| align="center" style="background:#f0f0f0;"|'''F primer'''
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| align="center" style="background:#f0f0f0;"|'''R primer'''
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| align="center" style="background:#f0f0f0;"|'''Expected length'''
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| align="center" style="background:#f0f0f0;"|'''Expected incorrect length'''
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| align="center" style="background:#f0f0f0;"|'''Product'''
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|-
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| Receiver int||P123||P112||2203||2430||Rec backbone
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|-
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| Receiver int||P119||P107||1051||||pLux
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|-
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| Receiver int||P125||P108||1051||||pRhl
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|-
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| Receiver int||P126||P109||1051||||pEsa
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|-
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| F2620||P113||P114||817||||LuxR
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|-
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| K092900+eCFP||P117||P118||765||||RhlR
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|-
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| EsaR synthesized||P121||P122||758||||EsaR
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|}
  
 
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Revision as of 15:04, 28 April 2014

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mm/dd/yyyy

PCR for Receiver assemblies

Template F primer R primer Expected length Expected incorrect length Product
Receiver int P123 P112 2203 2430 Rec backbone
Receiver int P119 P107 1051 pLux
Receiver int P125 P108 1051 pRhl
Receiver int P126 P109 1051 pEsa
F2620 P113 P114 817 LuxR
K092900+eCFP P117 P118 765 RhlR
EsaR synthesized P121 P122 758 EsaR