Difference between revisions of "Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/10/18"

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(mm/dd/yyyy)
(mm/dd/yyyy)
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Insert: Gblck pLac-RBS E/S: 2.6ng/ul  
 
Insert: Gblck pLac-RBS E/S: 2.6ng/ul  
 
:Cut, column purified<br>
 
:Cut, column purified<br>
"did a bunch of calculations, only actually tried 3:1 and bb ctrl''<br>
+
''did a bunch of calculations, only actually tried 3:1 and bb ctrl''<br>
 
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Revision as of 18:10, 21 October 2013

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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mm/dd/yyyy

Retry ligation with gel extracted J06702
Backbone: J06702 E/X: 44 ng/ul

Cut, gel purified

Insert: Gblck pLac-RBS E/S: 2.6ng/ul

Cut, column purified

did a bunch of calculations, only actually tried 3:1 and bb ctrl

1:1 2:1 3:1 Bb ctrl
insert 0.5 1.0 1.5 0
vector 1.13 1.13 1.13 1.13
2x Roche ligation buffer 5 5 5 5
T4 ligase 1 1 1 1
H20 2.37 1.87 1.37 2.87


ligations ran for 25min at RT
Incubate with 40ul DH5αT for 15 min on ice