Difference between revisions of "Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/09/28"

From OpenWetWare
Jump to: navigation, search
(mm/dd/yyyy)
(mm/dd/yyyy)
Line 25: Line 25:
 
|-
 
|-
 
|Ctrl||0.736||6645||
 
|Ctrl||0.736||6645||
| ||0.712||6485||
+
|-
 +
|||0.712||6485||
 
|-
 
|-
 
| ||0.740||6598||
 
| ||0.740||6598||
Line 46: Line 47:
 
<br>
 
<br>
 
spun receiver cells in 2mL tubes at 12k g for 5min
 
spun receiver cells in 2mL tubes at 12k g for 5min
 
+
<br>
 +
I didn't write this down but I'm assuming I split the receiver cells into the sender supernatant in singlet.
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
 
|}
 
|}
  
 
__NOTOC__
 
__NOTOC__

Revision as of 15:19, 14 October 2013

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

mm/dd/yyyy

8am

Sender/Receiver OD650 GFP RFP
EsaI 0.679 1500
0.671 1545
0.634 1402
RhlI 0.755 13497
0.792 14323
0.763 13992
Ctrl 0.736 6645
0.712 6485
0.740 6598
LuxR-GFP 0.548 1619
0.563 1858
0.576 1957
LuxR-RFP 0.576 768
0.564 682
0.562 694


Combined 3-5mL cultures of each sender into 15mL conicals. Spun in Wang lab large centrifuge
spun receiver cells in 2mL tubes at 12k g for 5min
I didn't write this down but I'm assuming I split the receiver cells into the sender supernatant in singlet.