Difference between revisions of "Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/09/28"

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(Autocreate 2013/09/28 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
 
(mm/dd/yyyy)
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==mm/dd/yyyy==
 
==mm/dd/yyyy==
* Insert content here...
+
'''8am'''
 
+
{| {{table}}
 +
|-
 +
|Sender/Receiver||OD650||GFP||RFP
 +
|-
 +
|EsaI||0.679||1500||
 +
|-
 +
| || 0.671||1545||
 +
|-
 +
| || 0.634||1402||
 +
|-
 +
|RhlI||0.755||13497||
 +
|-
 +
| || 0.792||14323||
 +
|-
 +
| || 0.763||13992||
 +
|-
 +
|Ctrl||0.736||6645||
 +
| ||0.712||6485||
 +
|-
 +
| ||0.740||6598||
 +
|-
 +
|LuxR-GFP||0.548||1619||
 +
|-
 +
|||0.563||1858||
 +
|-
 +
|||0.576||1957||
 +
|-
 +
|LuxR-RFP||0.576|| || 768
 +
|-
 +
| ||0.564 || ||682
 +
|-
 +
| ||0.562|| ||694
 +
|-
 +
|}
 +
<br>
 +
Combined 3-5mL cultures of each sender into 15mL conicals. Spun in Wang lab large centrifuge
 +
<br>
 +
spun receiver cells in 2mL tubes at 12k g for 5min
  
 
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Revision as of 15:16, 14 October 2013

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mm/dd/yyyy

8am

Sender/Receiver OD650 GFP RFP
EsaI 0.679 1500
0.671 1545
0.634 1402
RhlI 0.755 13497
0.792 14323
0.763 13992
Ctrl 0.736 6645 0.712 6485
0.740 6598
LuxR-GFP 0.548 1619
0.563 1858
0.576 1957
LuxR-RFP 0.576 768
0.564 682
0.562 694


Combined 3-5mL cultures of each sender into 15mL conicals. Spun in Wang lab large centrifuge
spun receiver cells in 2mL tubes at 12k g for 5min