Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/09/25: Difference between revisions
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Rene M Davis (talk | contribs) (Autocreate 2013/09/25 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs) |
Rene M Davis (talk | contribs) |
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==mm/dd/yyyy== | ==mm/dd/yyyy== | ||
{| {{table}} | |||
|- | |||
|Sender/Receiver||OD650||GFP||RFP||pH | |||
|- | |||
|EsaI 1 ||0.697||1034|| || 7-8 | |||
|- | |||
|EsaI ||0.678||1023|| || 7-8 | |||
|- | |||
|RhlI 1 ||0.707||6561|| || 7-8 | |||
|- | |||
|RhlI ||0.719||7153|| || 7-8 | |||
|- | |||
|Ctrl 1 ||0.737||5494|| || 7-8 | |||
|- | |||
|Ctrl ||0.692||5273|| || 7-8 | |||
|- | |||
|LuxR-GFP ||0.549||1510|| || | |||
|- | |||
|LuxR-RFP ||0.536|| ||748 || | |||
|- | |||
|} | |||
<br> | |||
Spun all cultures in 2mL tubes for 3min at 12.9RPM (15.4g). | |||
Moved sender sup to 15mL conical tubes (combined all 10mLs). | |||
3mL each sender into culture tubes. | |||
<br><br> | |||
Resuspended receivers into 150ul plain LB. | |||
moved 50ul each into 3mL each sender | |||
Started shaking at '''5PM''' | |||
<br><br> | |||
Extracted remaining 3mLs into 1mL ethyl acetate | |||
:15s vortex, 2min wait 5 times. 10 min wait last time | |||
''still didn't separate (all balled up on top of the media). added a second 1mL of ethyl acetate, repeated extraction protocol to get 1mL'' | |||
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__NOTOC__ | __NOTOC__ |
Revision as of 16:00, 14 October 2013
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mm/dd/yyyy
still didn't separate (all balled up on top of the media). added a second 1mL of ethyl acetate, repeated extraction protocol to get 1mL |