Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/09/08

From OpenWetWare
Revision as of 12:07, 12 September 2013 by Rene M Davis (talk | contribs) (mm/dd/yyyy)
Jump to: navigation, search
Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

mm/dd/yyyy

Redid PCR of all the reactions that didn't produce the desired product (see yesterday's chart) a lower annealing temp (50C)

Number Template Primer 1 Primer 2 Expected size Worked?
1 I13522 3 P060 P062 3136 No
2 K084007 12.9 P069 P070 645 Yes
3 K084007 18.3 P069 P070 645 No
4 K092900-E0420 80.5 P077 P078 765 Probably not
5 Part B1 P081 P082 980 No
6 Part B2 P081 P082 980 No
7 Part B3 P081 P082 980 No

GG PCR round 2, not the ugliest gel I've run