Difference between revisions of "Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/09/08"

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(mm/dd/yyyy)
(mm/dd/yyyy)
Line 11: Line 11:
 
{| {{table}}
 
{| {{table}}
 
|-
 
|-
|Number||Template||Primer 1||Primer 2||Expected size
+
|Number||Template||Primer 1||Primer 2||Expected size||Worked?
 
|-
 
|-
|1||I13522 3||P060||P062||3136
+
|1||I13522 3||P060||P062||3136|| No
 
|-
 
|-
|2||K084007 12.9||P069||P070||645
+
|2||K084007 12.9||P069||P070||645|| Yes
 
|-
 
|-
|3||K084007 18.3||P069||P070||645
+
|3||K084007 18.3||P069||P070||645|| No
 
|-
 
|-
|4||K092900-E0420 80.5||P077||P078||765
+
|4||K092900-E0420 80.5||P077||P078||765||Probably not
 
|-
 
|-
|5||Part B1||P081||P082||980
+
|5||Part B1||P081||P082||980|| No
 
|-
 
|-
|6||Part B2||P081||P082||980
+
|6||Part B2||P081||P082||980||No
 
|-
 
|-
|7||Part B3||P081||P082||980
+
|7||Part B3||P081||P082||980||No
 
|-
 
|-
 
|}
 
|}
 +
 +
[[image:13.09.09_GG_PCR_edited.png|600px|GG PCR round 2, not the ugliest gel I've run]]
 +
  
  

Revision as of 12:07, 12 September 2013

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mm/dd/yyyy

Redid PCR of all the reactions that didn't produce the desired product (see yesterday's chart) a lower annealing temp (50C)

Number Template Primer 1 Primer 2 Expected size Worked?
1 I13522 3 P060 P062 3136 No
2 K084007 12.9 P069 P070 645 Yes
3 K084007 18.3 P069 P070 645 No
4 K092900-E0420 80.5 P077 P078 765 Probably not
5 Part B1 P081 P082 980 No
6 Part B2 P081 P082 980 No
7 Part B3 P081 P082 980 No

GG PCR round 2, not the ugliest gel I've run