Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/09/06

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09/06/2013

PCR for Golden Gate assemblies

Number Template Primer 1 Primer 2 Expected size Worked?
1 I13522 73.4 P060 P062 3136 No
2 I13522 73.4 Px P084 3136 Yes
3 I13522 73.4 Py Pz 90 Yes
4 I13522 73.4 P060 P062 3136 No
5 I13522 2 Px P084 3136 Yes
6 I13522 2 Py Pz 90 Yes
7 I13522 73.4 P060 P062 3136 No
8 I13522 3 Px P084 3136 Yes
9 I13522 3 Py Pz 90 Yes
10 K145254 4 P063 P064 679 Yes
11 K145254 7/24/13 P063 P064 679 Yes
12 F2620+GFP 7/24/13 P065 P066 817 Yes
13 F2620+GFP 7/24/13 P067 P068 56 Yes
14 K084007 7/24/13 P069 P070 645 No
15 K082035 36 P075 P076 703 Yes
16 K092900-E0420 80.5 P077 P078 765 No
17 K092900-E0420 80.5 P079 P080 56 Yes
18 Part B1 P081 P082 980 No
19 Part B2 P081 P082 980 No
20 Part B3 P081 P082 980 No
21 PSB1C3 (1) P083 P084 3000 Yes
22 PSB1C3 (2) P083 P084 3000 Yes
23 I13522 2 P060 P062 3136 No
24 I13522 3 P060 P062 3136 No



GG PCR

GG PCR