Difference between revisions of "Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/09/06"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
 
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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| colspan="2"|
 
| colspan="2"|
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{| {{table}}
 
{| {{table}}
 
|-
 
|-
|Number||Template||Primer 1||Primer 2||Expected size
+
|Number||Template||Primer 1||Primer 2||Expected size||Worked?
 
|-
 
|-
|1||I13522 73.4||P060||P062||3136
+
|1||I13522 73.4||P060||P062||3136||No
 
|-
 
|-
|2||I13522 73.4||Px||P084||3136
+
|2||I13522 73.4||P095||P084||3136||Yes
 
|-
 
|-
|3||I13522 73.4||Py||Pz||90
+
|3||I13522 73.4||P093||P094||90||Yes
 
|-
 
|-
|4||I13522 73.4||P060||P062||3136
+
|4||I13522 73.4||P060||P062||3136||No
 
|-
 
|-
|5||I13522 2||Px||P084||3136
+
|5||I13522 2||P095||P084||3136||Yes
 
|-
 
|-
|6||I13522 2||Py||Pz||90
+
|6||I13522 2||P093||P094||90||Yes
 
|-
 
|-
|7||I13522 73.4||P060||P062||3136
+
|7||I13522 73.4||P060||P062||3136||No
 
|-
 
|-
|8||I13522 3||Px||P084||3136
+
|8||I13522 3||P095||P084||3136||Yes
 
|-
 
|-
|9||I13522 3||Py||Pz||90
+
|9||I13522 3||P093||P094||90||Yes
 
|-
 
|-
|10||K145254 4||P063||P064||679
+
|10||K145254 4||P063||P064||679||Yes
 
|-
 
|-
|11||K145254 7/24/13||P063||P064||679
+
|11||K145254 7/24/13||P063||P064||679||Yes
 
|-
 
|-
|12||F2620+GFP 7/24/13||P065||P066||817
+
|12||F2620+GFP 7/24/13||P065||P066||817||Yes
 
|-
 
|-
|13||F2620+GFP 7/24/13||P067||P068||56
+
|13||F2620+GFP 7/24/13||P067||P068||56||Yes
 
|-
 
|-
|14||K084007 7/24/13||P069||P070||645
+
|14||K084007 7/24/13||P069||P070||645||No
 
|-
 
|-
|15||K082035 36||P075||P076||703
+
|15||K082035 36||P075||P076||703||Yes
 
|-
 
|-
|16||K092900-E0420 80.5||P077||P078||765
+
|16||K092900-E0420 80.5||P077||P078||765||No
 
|-
 
|-
|17||K092900-E0420 80.5||P079||P080||56
+
|17||K092900-E0420 80.5||P079||P080||56||Yes
 
|-
 
|-
|18||Part B1||P081||P082||980
+
|18||Part B1||P081||P082||980||No
 
|-
 
|-
|19||Part B2||P081||P082||980
+
|19||Part B2||P081||P082||980||No
 
|-
 
|-
|20||Part B3||P081||P082||980
+
|20||Part B3||P081||P082||980||No
 
|-
 
|-
|21||PSB1C3 (1)||P083||P084||3000
+
|21||PSB1C3 (1)||P083||P084||3000||Yes
 
|-
 
|-
|22||PSB1C3 (2)||P083||P084||3000
+
|22||PSB1C3 (2)||P083||P084||3000||Yes
 
|-
 
|-
|23||I13522 2||P060||P062||3136
+
|23||I13522 2||P060||P062||3136||No
 
|-
 
|-
|24||I13522 3||P060||P062||3136
+
|24||I13522 3||P060||P062||3136||No
 
|-
 
|-
 
|}
 
|}

Latest revision as of 22:17, 26 September 2017

Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

09/06/2013

PCR for Golden Gate assemblies

Number Template Primer 1 Primer 2 Expected size Worked?
1 I13522 73.4 P060 P062 3136 No
2 I13522 73.4 P095 P084 3136 Yes
3 I13522 73.4 P093 P094 90 Yes
4 I13522 73.4 P060 P062 3136 No
5 I13522 2 P095 P084 3136 Yes
6 I13522 2 P093 P094 90 Yes
7 I13522 73.4 P060 P062 3136 No
8 I13522 3 P095 P084 3136 Yes
9 I13522 3 P093 P094 90 Yes
10 K145254 4 P063 P064 679 Yes
11 K145254 7/24/13 P063 P064 679 Yes
12 F2620+GFP 7/24/13 P065 P066 817 Yes
13 F2620+GFP 7/24/13 P067 P068 56 Yes
14 K084007 7/24/13 P069 P070 645 No
15 K082035 36 P075 P076 703 Yes
16 K092900-E0420 80.5 P077 P078 765 No
17 K092900-E0420 80.5 P079 P080 56 Yes
18 Part B1 P081 P082 980 No
19 Part B2 P081 P082 980 No
20 Part B3 P081 P082 980 No
21 PSB1C3 (1) P083 P084 3000 Yes
22 PSB1C3 (2) P083 P084 3000 Yes
23 I13522 2 P060 P062 3136 No
24 I13522 3 P060 P062 3136 No



GG PCR

GG PCR