Difference between revisions of "Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/09/06"

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==09/06/2013==
 
==09/06/2013==
PCR for Golden Gate assemblies<br> Decided to redo everything.
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PCR for Golden Gate assemblies
 
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[[image:13.09.07_GG_PCR_top_edited.png‎ |600px|GG PCR]]
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[[image:13.09.07_GG_PCR_bottom.png‎ |650px|GG PCR]]
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Revision as of 13:13, 12 September 2013

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09/06/2013

PCR for Golden Gate assemblies

Number Template Primer 1 Primer 2 Expected size
1 I13522 73.4 P060 P062 3136
2 I13522 73.4 Px P084 3136
3 I13522 73.4 Py Pz 90
4 I13522 73.4 P060 P062 3136
5 I13522 2 Px P084 3136
6 I13522 2 Py Pz 90
7 I13522 73.4 P060 P062 3136
8 I13522 3 Px P084 3136
9 I13522 3 Py Pz 90
10 K145254 4 P063 P064 679
11 K145254 7/24/13 P063 P064 679
12 F2620+GFP 7/24/13 P065 P066 817
13 F2620+GFP 7/24/13 P067 P068 56
14 K084007 7/24/13 P069 P070 645
15 K082035 36 P075 P076 703
16 K092900-E0420 80.5 P077 P078 765
17 K092900-E0420 80.5 P079 P080 56
18 Part B1 P081 P082 980
19 Part B2 P081 P082 980
20 Part B3 P081 P082 980
21 PSB1C3 (1) P083 P084 3000
22 PSB1C3 (2) P083 P084 3000
23 I13522 2 P060 P062 3136
24 I13522 3 P060 P062 3136



GG PCR

GG PCR