Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/07/19: Difference between revisions
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Rene M Davis (talk | contribs) (Autocreate 2013/07/19 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs) |
Rene M Davis (talk | contribs) |
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== | ==07/19/2013== | ||
Ligation/transformation plates have colonies but they're all in the middle. Same with the backbone control. Picked colonies just in case. <br> | |||
I03522 or whatever has colonies. Picked two. <br> | |||
Started the oligo annealing over again. I shouldn't have cut or dephosphorylated the oligos. That was dumb. <br> | |||
Miniprepped the colonies I picked last night. Tried splitting the 3ml cultures into 2-1.5ml cultures to mini prep. Still got low yields. | |||
{|table|} | |||
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|Part||Size||Miniprep yield 1||Miniprep yield 2 | |||
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|K092900+cyan||~2900|| | |||
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|F2620+GFP||~2000|| | |||
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|K082035|| || 6||8 | |||
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|K084007||835|| | |||
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|} | |||
<br> | |||
Cut a bunch of QS plasmids from the last few months with X/P to see which ones might be worth transforming and growing up to PCR out parts for the expression vectors. | |||
Revision as of 17:06, 19 July 2013
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07/19/2013Ligation/transformation plates have colonies but they're all in the middle. Same with the backbone control. Picked colonies just in case.
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