Difference between revisions of "Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/05/30"

From OpenWetWare
Jump to: navigation, search
(Entry title)
(Entry title)
Line 30: Line 30:
 
|TraR ctrl||LuxR
 
|TraR ctrl||LuxR
 
|-
 
|-
}}
+
|}
 
<br><br>
 
<br><br>
  

Revision as of 09:54, 30 May 2013

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Entry title

9AM

BL21 sender cultures grew in 6mls and 2mls. RhlR-GFP still didn't grow.
Shook LuxR, TraI, and TraR that had been in the fridge for a few minutes.

Co-cultures: Add 100ul of Sender, 100ul of Receiver to 3ml LB+Amp (except for TraI culture, which will not have antibiotic). Started shaking at 10AM.

Sender Receiver
LuxI LuxR
LasI LuxR
RhlI LuxR
TraI LuxR
TraR ctrl LuxR



Supernatant transfer: Determine pH using test strips:

Sender pH
LuxI
LasI
RhlI
TraI
TraR ctrl