Difference between revisions of "Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/05/30"

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(Autocreate 2013/05/30 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
 
(Entry title)
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==Entry title==
 
==Entry title==
* Insert content here...
+
9AM
 +
 
 +
BL21 sender cultures grew in 6mls and 2mls. RhlR-GFP still didn't grow.  
 +
<br>
 +
Shook LuxR, TraI, and TraR that had been in the fridge for a few minutes.  
 +
<br>
 +
<br>
 +
Co-cultures:
 +
Add 100ul of Sender, 100ul of Receiver to 3ml LB+Amp (except for TraI culture, which will not have antibiotic). Started shaking at 10AM.
 +
{| {{table}}
 +
|-
 +
|Sender||Receiver
 +
|-
 +
|LuxI||LuxR
 +
|-
 +
|LasI||LuxR
 +
|-
 +
|RhlI||LuxR
 +
|-
 +
|TraI||LuxR
 +
|-
 +
|TraR ctrl||LuxR
 +
|-
 +
}}
 +
<br><br>
 +
 
 +
Supernatant transfer:
 +
Determine pH using test strips:
 +
{| {{table}}
 +
|-
 +
|Sender||pH
 +
|-
 +
|LuxI||
 +
|-
 +
|LasI||
 +
|-
 +
|RhlI||
 +
|-
 +
|TraI||
 +
|-
 +
|TraR ctrl||
 +
|-
 +
|}
 +
 
 +
 
  
  

Revision as of 09:53, 30 May 2013

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Entry title

9AM

BL21 sender cultures grew in 6mls and 2mls. RhlR-GFP still didn't grow.
Shook LuxR, TraI, and TraR that had been in the fridge for a few minutes.

Co-cultures: Add 100ul of Sender, 100ul of Receiver to 3ml LB+Amp (except for TraI culture, which will not have antibiotic). Started shaking at 10AM.

}}

Supernatant transfer: Determine pH using test strips:
Sender Receiver
LuxI LuxR
LasI LuxR
RhlI LuxR
TraI LuxR
TraR ctrl LuxR
Sender pH
LuxI
LasI
RhlI
TraI
TraR ctrl