Difference between revisions of "Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/05/29"

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|Positive (Kind of)||GFP only (insert part #)
 
|Positive (Kind of)||GFP only (insert part #)

Revision as of 10:00, 29 May 2013

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Entry title

Set up experiment to test plate reader with GFP receivers. Started shaking at 11am.

Senders (6ml) Receivers (2ml)
LuxI LuxR-GFP
LasI
RhlI RhlR-GFP (maybe)
TraI
Controls
Positive (Kind of) GFP only (insert part #)
Negative sender TraR
Negative receiver none



Also picked TraI, TraR, and Ernesto's ligation of K092900-E0240 into BL21 for mini preps.