Difference between revisions of "Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/12"

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(Autocreate 2013/03/12 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
 
(Entry title)
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==Entry title==
 
==Entry title==
* Insert content here...
+
tonight:
 +
 
 +
miniprepped E0420 (E0343 didn't grow)
 +
 
 +
cut with X & P
 +
 
 +
cut K092900 with S & P
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 +
gel extracted
 +
 
 +
ligated
 +
 
 +
<br>
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ligation:
 +
 
 +
3.8ul backbone
 +
 
 +
13ul insert
 +
 
 +
behzad's roche buffer and ligase
 +
 
 +
not doing backbone control
 +
 
  
  

Revision as of 12:55, 28 May 2013

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Entry title

tonight:

miniprepped E0420 (E0343 didn't grow)

cut with X & P

cut K092900 with S & P

gel extracted

ligated


ligation:

3.8ul backbone

13ul insert

behzad's roche buffer and ligase

not doing backbone control