Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/08: Difference between revisions

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Revision as of 13:40, 28 May 2013

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Entry title

Ran the colony PCRs Ernesto ran yesterday on a gel:

colony PCR

Label Part # Part length Expected band length
plate 2 K082035 884bp 1134bp
plate 4 K084007 835bp 1085bp
plate 5 K081016 735bp 985bp
plate 7 K145254 862bp 1112bp

minipreps:

Label Part # colony part length expected band length
3-k R0071 picked by KAH 53bp 300bp
5-k K081016 picked by KAH 735bp 1035bp
2-2 K082035 colony 2 884bp 1134bp
4-2 K084007 colony 2 835bp 1085bp
7-1 K145254 colony 1 862bp 1112bp
7-3 K145254 colony 3 862bp 1112bp


Minipreps:

Part 260 280 260/280 ng/ul
B0034+C0061 0.042 0.022 1.873 41.772
B0034+C0061 0.043 0.023 1.866 43.094
B0034+C0078 0.061 0.031 1.93 60.539
B0034+C0078 0.031 0.017 1.832 31.39


Gel extractions:

Part 260 280 260/280 ng/ul
RBS+LuxI X/P 0.004 0.001 3.545 4.104
RBS+LasI X/P 0.002 0.001 2.125 1.809
R0071 S/P 0.006 0.003 2.069 6.317
K081023 X/P 0.025 0.014 1.859 25.325



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