Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/08: Difference between revisions
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Rene M Davis (talk | contribs) (Autocreate 2013/03/08 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs) |
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==Entry title== | ==Entry title== | ||
Ran the colony PCRs Ernesto ran yesterday on a gel: | |||
[[image:13.03.08_colony_PCR_(Ernesto)_edit.jpg|400px|colony PCR]] | |||
{| {{table}} | |||
|- | |||
|Label||Part #|| Part length|| Expected band length | |||
|- | |||
|plate 2|| K082035|| 884bp||1134bp | |||
|- | |||
|plate 4||K084007 ||835bp||1085bp | |||
|- | |||
|plate 5|| K081016|| 735bp||985bp | |||
|- | |||
|plate 7|| K145254|| 862bp||1112bp | |||
|} | |||
minipreps: | |||
{| {{table}} | |||
|- | |||
|Label||Part #|| colony|| part length || expected band length | |||
|- | |||
|3-k||R0071 ||picked by KAH ||53bp || 300bp | |||
|- | |||
|5-k||K081016|| picked by KAH|| 735bp||1035bp | |||
|- | |||
|2-2|| K082035 ||colony 2 ||884bp||1134bp | |||
|- | |||
|4-2|| K084007 ||colony 2 ||835bp||1085bp | |||
|- | |||
|7-1|| K145254 ||colony 1 ||862bp||1112bp | |||
|- | |||
|7-3|| K145254|| colony 3|| 862bp||1112bp | |||
|} | |||
<br> | |||
Minipreps: | |||
{| {{table}} | |||
|- | |||
|Part||260||280||260/280|| ng/ul | |||
|- | |||
|B0034+C0061||0.042||0.022||1.873||41.772 | |||
|- | |||
|B0034+C0061||0.043||0.023||1.866||43.094 | |||
|- | |||
|B0034+C0078||0.061||0.031||1.93||60.539 | |||
|- | |||
|B0034+C0078||0.031||0.017||1.832||31.39 | |||
|- | |||
|} | |||
<br> | |||
Gel extractions: | |||
{| {{table}} | |||
|- | |||
|Part||260||280||260/280|| ng/ul | |||
|- | |||
|RBS+LuxI X/P||0.004||0.001||3.545||4.104 | |||
|- | |||
|RBS+LasI X/P||0.002||0.001||2.125||1.809 | |||
|- | |||
|R0071 S/P||0.006||0.003||2.069||6.317 | |||
|- | |||
|K081023 X/P||0.025||0.014||1.859||25.325 | |||
|- | |||
|} | |||
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__NOTOC__ | __NOTOC__ |
Revision as of 13:40, 28 May 2013
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Entry titleRan the colony PCRs Ernesto ran yesterday on a gel:
minipreps:
|