Difference between revisions of "Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/08"

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(Autocreate 2013/03/08 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
 
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==Entry title==
 
==Entry title==
* Insert content here...
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Ran the colony PCRs Ernesto ran yesterday on a gel:
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[[image:13.03.08_colony_PCR_(Ernesto)_edit.jpg‎|400px|colony PCR]]
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{| {{table}}
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|-
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|Label||Part #|| Part length|| Expected band length
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|-
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|plate 2|| K082035|| 884bp||1134bp
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|-
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|plate 4||K084007 ||835bp||1085bp
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|-
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|plate 5|| K081016|| 735bp||985bp
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|-
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|plate 7|| K145254|| 862bp||1112bp
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|}
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minipreps:
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{| {{table}}
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|-
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|Label||Part #|| colony|| part length || expected band length
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|-
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|3-k||R0071 ||picked by KAH ||53bp || 300bp
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|-
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|5-k||K081016|| picked by KAH|| 735bp||1035bp
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|-
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|2-2|| K082035 ||colony 2 ||884bp||1134bp
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|-
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|4-2|| K084007 ||colony 2 ||835bp||1085bp
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|-
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|7-1|| K145254 ||colony 1 ||862bp||1112bp
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|-
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|7-3|| K145254|| colony 3|| 862bp||1112bp
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|}
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<br>
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Minipreps:
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{| {{table}}
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|-
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|Part||260||280||260/280|| ng/ul
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|-
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|B0034+C0061||0.042||0.022||1.873||41.772
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|-
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|B0034+C0061||0.043||0.023||1.866||43.094
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|-
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|B0034+C0078||0.061||0.031||1.93||60.539
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|-
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|B0034+C0078||0.031||0.017||1.832||31.39
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|-
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|}
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<br>
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Gel extractions:
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{| {{table}}
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|-
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|Part||260||280||260/280|| ng/ul
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|-
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|RBS+LuxI X/P||0.004||0.001||3.545||4.104
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|-
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|RBS+LasI X/P||0.002||0.001||2.125||1.809
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|-
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|R0071 S/P||0.006||0.003||2.069||6.317
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|-
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|K081023 X/P||0.025||0.014||1.859||25.325
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|-
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|}
  
  
 
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Revision as of 13:40, 28 May 2013

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Entry title

Ran the colony PCRs Ernesto ran yesterday on a gel:

colony PCR

Label Part # Part length Expected band length
plate 2 K082035 884bp 1134bp
plate 4 K084007 835bp 1085bp
plate 5 K081016 735bp 985bp
plate 7 K145254 862bp 1112bp

minipreps:

Label Part # colony part length expected band length
3-k R0071 picked by KAH 53bp 300bp
5-k K081016 picked by KAH 735bp 1035bp
2-2 K082035 colony 2 884bp 1134bp
4-2 K084007 colony 2 835bp 1085bp
7-1 K145254 colony 1 862bp 1112bp
7-3 K145254 colony 3 862bp 1112bp


Minipreps:

Part 260 280 260/280 ng/ul
B0034+C0061 0.042 0.022 1.873 41.772
B0034+C0061 0.043 0.023 1.866 43.094
B0034+C0078 0.061 0.031 1.93 60.539
B0034+C0078 0.031 0.017 1.832 31.39


Gel extractions:

Part 260 280 260/280 ng/ul
RBS+LuxI X/P 0.004 0.001 3.545 4.104
RBS+LasI X/P 0.002 0.001 2.125 1.809
R0071 S/P 0.006 0.003 2.069 6.317
K081023 X/P 0.025 0.014 1.859 25.325