Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/02

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Did tons of minipreps, many of them did not work well.

Probably picked colonies from the new plates

Cut some parts and ran a gel of cut plasmids:


Lane label Part number Restriction enzymes expected length
1 F2620 X & P 1061 & 2079
2 constitutive promoter S & P 2000ish
3 constitutive promoter w RFP S&P 2000ish
4 R0071 (probably) S & P 3029
5 K081016 X & P 735 & 2079
6 K081023 X & P 1653 & 2079