Difference between revisions of "Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/02"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
 
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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==Entry title==
 
==Entry title==
* Insert content here...
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Did tons of minipreps, many of them did not work well.
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Probably picked colonies from the new plates
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Cut some parts and ran a gel of cut plasmids:
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[[image:13.03.02_edit.jpg‎ |400px|digests]]
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{| {{table}}
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|-
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|Lane label || Part number || Restriction enzymes||expected length
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|-
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|1||F2620|| X & P|| 1061 & 2079
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|-
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|2||constitutive promoter||S & P ||2000ish
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|-
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|3||constitutive promoter w RFP||S&P||2000ish
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|-
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|4|| R0071 (probably) || S & P || 3029
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|-
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|5||K081016|| X & P || 735 & 2079
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|-
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||6|| K081023|| X & P || 1653 & 2079
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|}
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Latest revision as of 22:32, 26 September 2017

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Entry title

Did tons of minipreps, many of them did not work well.

Probably picked colonies from the new plates

Cut some parts and ran a gel of cut plasmids:

digests

Lane label Part number Restriction enzymes expected length
1 F2620 X & P 1061 & 2079
2 constitutive promoter S & P 2000ish
3 constitutive promoter w RFP S&P 2000ish
4 R0071 (probably) S & P 3029
5 K081016 X & P 735 & 2079
6 K081023 X & P 1653 & 2079