Haynes Lab:Notebook/CRISPR Editing/2014/08/07: Difference between revisions
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* Designed nested PCR primers to gradually build homology arms onto mCherry. The resulting donor should... | * Designed nested PCR primers to gradually build homology arms onto mCherry. The resulting donor should... | ||
** Place mCherry in-frame upstream of the luciferase gene | ** Place mCherry in-frame upstream of the luciferase gene | ||
** Add an | ** Add an ATG start codon onto mCherry (we had to use KAH154 as a template, where mCherry has no start) | ||
** Add a double stop (TAATAG) onto the end of mCherry | |||
** Add 130 bp of homology with TK-luciferase to each end | |||
Revision as of 08:58, 7 August 2014
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08/07/2014
CRISPR on chromatin: transfections
Luc-mCh swap: building the donor sequence
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