Haynes Lab:Notebook/CRISPR Editing/2014/07/04: Difference between revisions
Rene M Davis (talk | contribs) |
Rene M Davis (talk | contribs) |
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Digest of pX330 and pX335. 4ug of each in 80ul reaction, 4ul BbsI. Left at 37°C for about 25 minutes. gel extracted<br><br> | Digest of pX330 and pX335. 4ug of each in 80ul reaction, 4ul BbsI. Left at 37°C for about 25 minutes. gel extracted<br><br> | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Sample Read#''' | |||
| align="center" style="background:#f0f0f0;"|'''260''' | |||
| align="center" style="background:#f0f0f0;"|'''280''' | |||
| align="center" style="background:#f0f0f0;"|'''260/280''' | |||
| align="center" style="background:#f0f0f0;"|'''ng/µL''' | |||
| align="center" style="background:#f0f0f0;"|'''total ug''' | |||
| align="center" style="background:#f0f0f0;"|'''%recovery''' | |||
|- | |||
| pX330 ||3.70E-02||1.80E-02||2.052||36.788||1.8394||0.45985 | |||
|- | |||
| pX335||4.70E-02||2.40E-02||1.918||46.529||2.32645||0.5816125 | |||
|} | |||
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also ran mCh donor sequence on the gel, didn't see any bands but it's possible I didn't have enough EtBr because all my bands were faint. The bands should be pretty small so even though they're PCR products, they still might be faint. Didn't think to soak the gel. will rerun<br><br> | also ran mCh donor sequence on the gel, didn't see any bands but it's possible I didn't have enough EtBr because all my bands were faint. The bands should be pretty small so even though they're PCR products, they still might be faint. Didn't think to soak the gel. will rerun<br><br> | ||
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Revision as of 14:55, 8 July 2014
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Entry titleAnnealing gRNA oligos 3ul 100uM top oligo 20 ul reactions
Digest of pX330 and pX335. 4ug of each in 80ul reaction, 4ul BbsI. Left at 37°C for about 25 minutes. gel extracted
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