Haynes Lab:Notebook/ASU iGEM/2012/06/15

From OpenWetWare
Revision as of 17:32, 3 July 2012 by Karmella Haynes (talk | contribs) (mm-dd-yy)
Jump to: navigation, search
Owwnotebook icon.pngASU iGEM 2012 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

6-15-12

  • Competent cell prep
  • Centrifuged falcon test tubes containing liquid colonies
  • Resuspended in CaCl2 buffer solution and incubated for 15 mins
  • Centrifuged and resuspended in CaCl2 glycerol buffer solution
  • Chilled overnight