Difference between revisions of "Haynes Lab:Notebook/ASU iGEM"

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==Project Description/Abstract==
 
==Project Description/Abstract==
  
[[#THAT| '''Team Chimeric Reporter:''']]<br>
+
'''Team Chimeric Reporter'''<br>
* Hyder Hussain
+
* Hyder Hussain: Topo target plasmid design and building
* Abhinav Markus
+
* Abhinav Markus: (new) Split beta gal assay development for the chimeric reporter (biotinylation proof of concept model)
* Ryan Muller
+
* Maddie Sands: (new) Split beta gal assay development for the chimeric reporter (biotinylation proof of concept model)
* Ethan Ward
+
* Ryan Muller: Inducible Topo chimeric protein production
[[#THIS| '''Team Cell Surface Reporter:''']]<br>
+
* Ethan Ward: Inducible Topo chimeric protein production
* Nisarg Patel
+
 
* Ryan Muller
+
 
* Rohit Rajan
+
'''Team Cell Surface Reporter'''<br>
* Ellen Qin
+
* Nisarg Patel: Split beta gal assay development for the magainin reporter
* Melinda Jenner
+
* Ellen Qin: Magainin fusion protein BioBricking
* Joe Barth
+
 
 +
 
 +
'''Support'''<br>
 +
* Rohit Rajan: Buffers, media (and quality control), preparation of BioBrick registry submissions
 +
* Melinda Jenner: Buffers, media (and quality control), preparation of BioBrick registry submissions
 +
* Amanda Ispas: Buffers, media (and quality control), preparation of BioBrick registry submissions
 +
 
  
 
==BioBricks==
 
==BioBricks==
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*14. His-Tag + 10aa GS Linker + Bgal fragment (1-w)
 
*14. His-Tag + 10aa GS Linker + Bgal fragment (1-w)
  
==Notes==
 
 
 
 
 
===6-15-12===
 
*Competent cell prep
 
:*Centrifuged falcon test tubes containing liquid colonies
 
:*Resuspended in CaCl<sub>2</sub> buffer solution and incubated for 15 mins
 
:*Centrifuged and resuspended in CaCl<sub>2</sub> glycerol buffer solution
 
:*Chilled overnight
 
  
 +
==Science Resources==
 +
* [http://biologylabs.utah.edu/jorgensen/wayned/ape/ A Plasmid Editor (ApE)]: freeware for viewing, editing, annotating, translating, and finding restriction sites in DNA sequences
 +
* [http://gcat.davidson.edu/igem10/ Oligator]: tool for creating BioBricks using oligo assembly
 +
* [http://www.rcsb.org/pdb/home/home.do Protein Data Bank (PDB)]: search for, view, and download full atomic coordinate data for 3D structures of proteins and other molecules. Get tips on editing Jmol 3D images at the [http://openwetware.org/wiki/Haynes:Jmol_Guide Haynes Lab OWW site].
 +
* [http://openwetware.org/wiki/Haynes:Protocols Protocols - Haynes Lab]: some protocols from the Haynes Lab
  
 +
==General Lab Links==
 +
* [https://docs.google.com/spreadsheet/ccc?key=0AghpgNgJLx-ldGtGSUhRRjFRQ0VCdlo4WXdyNlVwWVE Ordering Spreadsheet]
  
 +
==Safety==
 +
* [https://cfo.asu.edu/ehs Environmental Health & Safety at ASU]
 +
* [http://ehsaweb.asu.edu/ Individual Training Record]: To check your ASU individual safety training record, log in at the EHS Assistant page. Click the "Individual Training Record" link and then enter your ASU ID.
  
  
 
__NOTOC__
 
__NOTOC__

Revision as of 23:13, 16 August 2012

<!-- sibboleth --><div id="lncal1" style="border:0px;"><div style="display:none;" id="id">lncal1</div><div style="display:none;" id="dtext">06/07/2012,06/08/2012,06/12/2012,06/13/2012,06/14/2012,06/15/2012,06/16/2012,06/17/2012,06/19/2012,06/20/2012,06/21/2012,06/22/2012,06/26/2012,06/27/2012,06/28/2012,07/02/2012,07/03/2012,07/24/2012,07/25/2012,07/26/2012,07/27/2012,07/28/2012,07/30/2012,07/31/2012,08/03/2012,08/10/2012,08/13/2012,08/14/2012,08/15/2012,08/16/2012</div><div style="display:none;" id="page">Haynes Lab:Notebook/ASU iGEM</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month"></div><div style="display:none;" id="year"></div><div style="display:none;" id="readonly">Y</div></div>

Owwnotebook icon.png ASU iGEM 2012 <sitesearch>title=Search this Project</sitesearch>

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Project Description/Abstract

Team Chimeric Reporter

  • Hyder Hussain: Topo target plasmid design and building
  • Abhinav Markus: (new) Split beta gal assay development for the chimeric reporter (biotinylation proof of concept model)
  • Maddie Sands: (new) Split beta gal assay development for the chimeric reporter (biotinylation proof of concept model)
  • Ryan Muller: Inducible Topo chimeric protein production
  • Ethan Ward: Inducible Topo chimeric protein production


Team Cell Surface Reporter

  • Nisarg Patel: Split beta gal assay development for the magainin reporter
  • Ellen Qin: Magainin fusion protein BioBricking


Support

  • Rohit Rajan: Buffers, media (and quality control), preparation of BioBrick registry submissions
  • Melinda Jenner: Buffers, media (and quality control), preparation of BioBrick registry submissions
  • Amanda Ispas: Buffers, media (and quality control), preparation of BioBrick registry submissions


BioBricks

Magainin Reporter

  • Magainin
  • Magainin + 2xGS Linker + His-Tag
  • Bgal fragment (a-1)
  • Bgal fragment (a-4)
  • Bgal fragment (w)
  • Bgal fragment (1-w)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (a-1)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (a-4)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (w)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (1-w)
  • His-Tag + 10aa GS Linker + Bgal fragment (a-1)
  • His-Tag + 10aa GS Linker + Bgal fragment (a-4)
  • 13. His-Tag + 10aa GS Linker + Bgal fragment (w)
  • 14. His-Tag + 10aa GS Linker + Bgal fragment (1-w)


Science Resources

General Lab Links

Safety