Difference between revisions of "Haynes Lab:Notebook/ASU iGEM"

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:* Plated 2 copies of each (100 ul, 250 ul) on LB amp plates.
 
:* Plated 2 copies of each (100 ul, 250 ul) on LB amp plates.
 
* Made 50 LB Amp plates.
 
* Made 50 LB Amp plates.
 
===6-20-12===
 
* Plated negative control on LB Amp plate
 
* Liquid cultures of T7 promoter and constitutive promoter
 
*Transformation (LSE)
 
:*Transformed DNA:
 
::*RBS (well 1:1H BBa_B0030)
 
::*TetR GFP (well 2:8A Part:BBa_I13522)
 
:* Cells: DH5 alpha (donated)
 
:* Protocol from: http://tools.invitrogen.com/content/sfs/manuals/subcloningefficiencydh5alpha_man.pdf (30 minute transformation)
 
:* Controls: puc19, no DNA
 
  
  

Revision as of 17:26, 3 July 2012

<!-- sibboleth --><div id="lncal1" style="border:0px;"><div style="display:none;" id="id">lncal1</div><div style="display:none;" id="dtext">06/07/2012,06/08/2012,06/12/2012,06/13/2012,06/14/2012,06/15/2012,06/16/2012,06/17/2012,06/19/2012,06/20/2012,06/21/2012,06/22/2012,06/26/2012,06/27/2012,06/28/2012,07/02/2012,07/03/2012,07/24/2012,07/25/2012,07/26/2012,07/27/2012,07/28/2012,07/30/2012,07/31/2012,08/03/2012,08/10/2012,08/13/2012,08/14/2012,08/15/2012,08/16/2012</div><div style="display:none;" id="page">Haynes Lab:Notebook/ASU iGEM</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month"></div><div style="display:none;" id="year"></div><div style="display:none;" id="readonly">Y</div></div>

Owwnotebook icon.png ASU iGEM 2012 <sitesearch>title=Search this Project</sitesearch>

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Project Description/Abstract

Team Chimeric Reporter:

  • Hyder Hussain
  • Abhinav Markus
  • Ryan Muller
  • Ethan Ward

Team Cell Surface Reporter:

  • Nisarg Patel
  • Ryan Muller
  • Rohit Rajan
  • Ellen Qin
  • Melinda Jenner
  • Joe Barth

BioBricks

Magainin Reporter

  • Magainin
  • Magainin + 2xGS Linker + His-Tag
  • Bgal fragment (a-1)
  • Bgal fragment (a-4)
  • Bgal fragment (w)
  • Bgal fragment (1-w)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (a-1)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (a-4)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (w)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (1-w)
  • His-Tag + 10aa GS Linker + Bgal fragment (a-1)
  • His-Tag + 10aa GS Linker + Bgal fragment (a-4)
  • 13. His-Tag + 10aa GS Linker + Bgal fragment (w)
  • 14. His-Tag + 10aa GS Linker + Bgal fragment (1-w)

Notes

6-15-12

  • Competent cell prep
  • Centrifuged falcon test tubes containing liquid colonies
  • Resuspended in CaCl2 buffer solution and incubated for 15 mins
  • Centrifuged and resuspended in CaCl2 glycerol buffer solution
  • Chilled overnight

6-16-12

  • Competent cell prep
  • Aliquotted 200uL into test tubes
  • Stored in -80C

6-17-12

  • Streak plated prepared competent cells on LB no amp plate
  • Colonies observed

6-19-12

  • Transformation (LSE)
  • Transformed DNA:
  • T7 promoter BBa_I712074
  • Constitutive promoter BBa_J23102
  • Made 50 LB Amp plates.