Haynes:WikiTemp NBplasmids: Difference between revisions
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| Plasmid || OD260 || OD260/280 || ng/μL | | Plasmid || OD260 || OD260/280 || ng/μL | ||
|- | |- | ||
| 1. Plasmid 1 || | | 1. Plasmid 1 || --- || --- || --- | ||
|- | |- | ||
| 2. Plasmid 2 || | | 2. Plasmid 2 || --- || --- || --- | ||
|} | |} | ||
'''Restriction Digest Table'''<br> | |||
* Checked plasmid minipreps with EcoRI/PstI digests | |||
{| {{table}} border="1" cellspacing="3" | |||
<!-- Editing: the coding for this table is a bit more advanced. --> | |||
<!-- valign="top" aligns all the text in the first row to the top. --> | |||
<!-- The | symbols on the next two lines start new cells in the same row. This does the same thing as ||, but you have to use | on new lines to set formatting for cells. --> | |||
<!-- bgcolor=#cfcfcf *colors* the *background* of the Reagent and Volume cells grey. --> | |||
<!-- The next two "rowspan=7" cells span all 7 rows in the table so that they can fit the "Expected" list and a gel image. rowspan is how you create merged rows in Wiki code. --> | |||
<!-- Replace Plasmid 1 and 2 with the names of your plasmids. Replace insert size and vector size with appropriate information for your plasmids. If you only have one Plasmid, delete all the text for Plasmid 2 | |||
<!-- After you upload your gel image to OWW, replace "GelImage.jpg" with the name of your image file --> | |||
<!-- is ASCII code for an invisible space. --> | |||
|- valign="top" | |||
| bgcolor=#cfcfcf | Reagent | |||
| bgcolor=#cfcfcf | Volume | |||
| rowspan="7" | <u>Expected:</u><br>1. Plasmid 1 = insert size, vector size<br>2. Plasmid 2 = insert size, vector size<br> | |||
| rowspan="7" | [[Image:GelImage.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | |||
|- | |||
| DNA(plasmid) || 3.0 μL | |||
|- | |||
| 10X buffer || 1.5 | |||
|- | |||
| EcoRI || 1.0 | |||
|- | |||
| PstI || 1.0 | |||
|- | |||
| dH<sub>2</sub>O || 8.5 | |||
|- | |||
| || 15 μL --> 37°C/ 15 min. | |||
|} | |||
'''DNA Sequencing Samples''' | |||
<!-- * signs create an automatically bulleted list. Replace 'date' with the date you submitted the samples --> | |||
<!-- The Order Number is shown at the top of your sequencing order form. Record in the indicated spot below --> | |||
* Submitted to DNASU on: 'date' | |||
* Order Number: | |||
<!-- # signs create an automatically numbered list. Replace Plasmid 1 & 2 with the names of your plasmids. Replace 'name' with the appropriate name of each primer. --> | |||
# Plasmid 1 - forward primer 'name' | |||
# Plasmid 1 - reverse primer 'name' | |||
# Plasmid 2 - forward primer 'name' | |||
# Plasmid 2 - reverse primer 'name' | |||
<!--TEMPLATE CODE--> | <!--TEMPLATE CODE--> | ||
Latest revision as of 14:57, 21 September 2013
< Haynes Lab OWW Home | Wiki Editing
Plasmid Validation Template page
DNA Concentration Data
| Plasmid | OD260 | OD260/280 | ng/μL |
| 1. Plasmid 1 | --- | --- | --- |
| 2. Plasmid 2 | --- | --- | --- |
Restriction Digest Table
- Checked plasmid minipreps with EcoRI/PstI digests
| Reagent | Volume | Expected: 1. Plasmid 1 = insert size, vector size 2. Plasmid 2 = insert size, vector size |
 15 μL/lane; 1% agarose; Ladder |
| DNA(plasmid) | 3.0 μL | ||
| 10X buffer | 1.5 | ||
| EcoRI | 1.0 | ||
| PstI | 1.0 | ||
| dH2O | 8.5 | ||
| 15 μL --> 37°C/ 15 min. |
DNA Sequencing Samples
- Submitted to DNASU on: 'date'
- Order Number:
- Plasmid 1 - forward primer 'name'
- Plasmid 1 - reverse primer 'name'
- Plasmid 2 - forward primer 'name'
- Plasmid 2 - reverse primer 'name'
