Haynes:WikiTemp NBplasmids: Difference between revisions
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< [http://openwetware.org/wiki/Haynes_Lab Haynes Lab OWW Home] | [http://openwetware.org/wiki/Haynes:TechResources#Wiki_Editing Wiki Editing] | |||
=Plasmid Validation Template page= | |||
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'''DNA Sequencing Samples''' | '''DNA Sequencing Samples''' | ||
<!-- * signs create an automatically bulleted list. Replace 'date' with the date you submitted the samples --> | <!-- * signs create an automatically bulleted list. Replace 'date' with the date you submitted the samples --> | ||
* Submitted to DNASU on 'date' | <!-- The Order Number is shown at the top of your sequencing order form. Record in the indicated spot below --> | ||
* Submitted to DNASU on: 'date' | |||
* Order Number: | |||
<!-- # signs create an automatically numbered list. Replace Plasmid 1 & 2 with the names of your plasmids. Replace 'name' with the appropriate name of each primer. --> | <!-- # signs create an automatically numbered list. Replace Plasmid 1 & 2 with the names of your plasmids. Replace 'name' with the appropriate name of each primer. --> | ||
# Plasmid 1 - forward primer 'name' | # Plasmid 1 - forward primer 'name' | ||
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# Plasmid 2 - forward primer 'name' | # Plasmid 2 - forward primer 'name' | ||
# Plasmid 2 - reverse primer 'name' | # Plasmid 2 - reverse primer 'name' | ||
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Latest revision as of 14:57, 21 September 2013
< Haynes Lab OWW Home | Wiki Editing
Plasmid Validation Template page
DNA Concentration Data
| Plasmid | OD260 | OD260/280 | ng/μL |
| 1. Plasmid 1 | --- | --- | --- |
| 2. Plasmid 2 | --- | --- | --- |
Restriction Digest Table
- Checked plasmid minipreps with EcoRI/PstI digests
| Reagent | Volume | Expected: 1. Plasmid 1 = insert size, vector size 2. Plasmid 2 = insert size, vector size |
 15 μL/lane; 1% agarose; Ladder |
| DNA(plasmid) | 3.0 μL | ||
| 10X buffer | 1.5 | ||
| EcoRI | 1.0 | ||
| PstI | 1.0 | ||
| dH2O | 8.5 | ||
| 15 μL --> 37°C/ 15 min. |
DNA Sequencing Samples
- Submitted to DNASU on: 'date'
- Order Number:
- Plasmid 1 - forward primer 'name'
- Plasmid 1 - reverse primer 'name'
- Plasmid 2 - forward primer 'name'
- Plasmid 2 - reverse primer 'name'
