Difference between revisions of "Haynes:WikiTemp NBplasmids"

From OpenWetWare
Jump to: navigation, search
Line 5: Line 5:
 
<!--TEMPLATE CODE-->
 
<!--TEMPLATE CODE-->
  
'''DNA Concentration Data'''<br>
+
'''DNA Concentration Data'''
 +
 
 +
<!-- Wiki code notes: The {| is the opening tag for tables. {{table}} is an OWW template that creates thin grey border lines and cell margins.
 +
<!-- Each |- starts a new row. There are three rows. bgcolor=#cfcfcf *colors* the *background* of the first row grey.  -->
 +
<!-- Below that, each | starts a row of cells. -->
 +
<!-- The symbol || separates cells in a row. Replace the --- in each cell with your data. -->
 +
<!-- |} must be the last symbol (on its own line) in the table. This is the table closing tag. -->
  
 
{| {{table}} cellspacing="3"  
 
{| {{table}} cellspacing="3"  
<!-- Each |- starts a new row. -->
 
<!-- Below that, each | starts a row of cells. -->
 
<!-- The symbol || separates cells in the table. Replace the --- in each cell with your data. -->
 
<!-- |} must be the last symbol in the table. This is the table closing tag. -->
 
 
|- bgcolor=#cfcfcf  
 
|- bgcolor=#cfcfcf  
 
| Plasmid || OD260 || OD260/280 || ng/μL
 
| Plasmid || OD260 || OD260/280 || ng/μL

Revision as of 07:42, 3 September 2013


DNA Concentration Data


Plasmid OD260 OD260/280 ng/μL
1. Plasmid 1 --- --- ---
2. Plasmid 2 --- --- ---


Restriction Digest Table

  • Check plasmid minipreps with EcoRI/PstI digests
Reagent Volume Expected:
1. Plasmid 1 = insert size, vector size
2. Plasmid 2 = insert size, vector size
Today's gel
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 8.5
  15 μL --> 37°C/ 15 min.