Haynes:TRIzol RNeasy

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Revision as of 10:41, 7 January 2013 by Karmella Haynes (talk | contribs) (New page: <- [http://openwetware.org/wiki/Haynes:Protocols Back to Protocols] '''RNA Miniprep - TRIzol/ RNeasy Sping Column Combo'''<br> by Karmella Haynes, 2013 Principle: LYSE CELLS IN TRI...)
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RNA Miniprep - TRIzol/ RNeasy Sping Column Combo
by Karmella Haynes, 2013


Principle:


LYSE CELLS IN TRIZOL

  1. Suspension cells: Add up to 1x10^6 cells to a 2.0 mL microfuge tube. Spin for 5 min. at 800 xg (~3000 rpm in a standard table top centrifuge) at room temp. Discard supernatant and add 500 m TRIzol (per . Mix by pipetting up and down. Incubate at room temperature for 5 min. Store at -80°C or go on to the next step.
  2. Adherent cells:

EXTRACT RNA - PHASE SEPARATION

  1. Add 200 μL chloroform per 1 mL TRIzol (