Haynes:Protocols

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Protocols

BioBrick Assembly

Cell Culture: Bacteria

Cell Culture: Mammalian

  • Coming soon

Resources at OpenWetWare

  • Materials: useful buffer formulas and specific information on commonly used reagents, such as LB broth, ampicillin, restriction enzymes, etc.




Recipes

LB Broth (Lennox)  
Caesin tryptone* 10 g
Yeast extract* 5 g
NaCl* 5 g
1 N NaOH 1 mL
  1000 mL
  • Dissolve in 1000 mL dH2O. Autoclave to sterilize.
  • Cool to room temperature before adding antibiotics
* Note: Can use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone,  yeast extract, and NaCl.