Difference between revisions of "Haynes:Protocols"

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(Protocols)
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'''Cell Culture: Mammalian'''
 
'''Cell Culture: Mammalian'''
 
* Coming soon
 
* Coming soon
 +
 +
'''Resources at OpenWetWare'''
 +
* [http://openwetware.org/wiki/Materials Materials]: useful buffer formulas and specific information on commonly used reagents, such as LB broth, ampicillin, restriction enzymes, etc.
  
  

Revision as of 10:21, 19 January 2012


Protocols

BioBrick Assembly

Cell Culture: Bacteria

Cell Culture: Mammalian

  • Coming soon

Resources at OpenWetWare

  • Materials: useful buffer formulas and specific information on commonly used reagents, such as LB broth, ampicillin, restriction enzymes, etc.




Recipes

LB Broth (Lennox)  
Caesin tryptone* 10 g
Yeast extract* 5 g
NaCl* 5 g
1 N NaOH 1 mL
  1000 mL
  • Dissolve in 1000 mL dH2O. Autoclave to sterilize.
  • Cool to room temperature before adding antibiotics
* Note: Can use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone,  yeast extract, and NaCl.