Haynes:MamCultureMedia

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Revision as of 16:25, 27 October 2012 by Karmella Haynes (talk | contribs) (New page: {| class="wikitable" |- | '''Cell line''' || '''Incomplete media''' || '''Supplements for complete media (required)''' || '''Transgene selection''' |- | U2OS (adherent) || McCoy's 5A || 10...)
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Cell line Incomplete media Supplements for complete media (required) Transgene selection
U2OS (adherent) McCoy's 5A 10% FBS, 1 % pen/strep ---
K-562 (suspension)


  • FBS = fetal bovine serum
  • FBS-tf = tetracycline-free FBS (required to maintain the "off" state of dox-inducible transgenes)
  • pen/strep = penicillin and streptomycin anti fungal supplement
  • Filter sterilize all media before use
  • Store all media at 4°C