Difference between revisions of "Haynes:MamCultureMedia"

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(Transgenic Cells)
("Wild type" stock cells (non-transgenic))
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=="Wild type" stock cells (non-transgenic)==
 
=="Wild type" stock cells (non-transgenic)==
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{| class="wikitable" cellspacing=5px
 
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| '''Cell line''' || '''Incomplete media''' || '''Supplements for complete media (required)'''
 
| '''Cell line''' || '''Incomplete media''' || '''Supplements for complete media (required)'''
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| U2OS (adherent) || McCoy's 5A || 10% FBS, 1 % pen/strep  
 
| U2OS (adherent) || McCoy's 5A || 10% FBS, 1 % pen/strep  
 
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|}
 
  
 
==Transgenic Cells==
 
==Transgenic Cells==

Revision as of 15:45, 27 October 2012

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Mammalian Culture Media

"Wild type" stock cells (non-transgenic)

Cell line Incomplete media Supplements for complete media (required)
K-562 (suspension) Iscove's Modified Dulbecco's Medium (IMDM) 10% FBS, 1 % pen/strep
SK-N-SH (adherent) Eagle's Minimum Essential Medium (EMEM) 10% FBS, 1 % pen/strep
U2OS (adherent) McCoy's 5A 10% FBS, 1 % pen/strep

Transgenic Cells

Cell Type Genotype Incomplete media Supplements for complete media (required) Antibiotic(s) for maintenance Antibiotic(s) for selection
U2OS (adherent) 126-1 McCoy's 5A 10% FBS, 1 % pen/strep ## ug/mL puromycin ## ug/mL puromycin

Notes

  • FBS = fetal bovine serum
  • FBS-tf = tetracycline-free FBS (required to maintain the "off" state of dox-inducible transgenes)
  • pen/strep = penicillin and streptomycin anti fungal supplement
  • Filter sterilize all media before use
  • Store all media at 4°C