Difference between revisions of "Haynes:MamCultureMedia"

From OpenWetWare
Jump to: navigation, search
Line 1: Line 1:
 +
<- [[Haynes:Protocols | Back to Protocols]]
 +
 +
<div style="width: 1000px">
 +
 
=Mammalian Culture Media=
 
=Mammalian Culture Media=
  
Line 19: Line 23:
  
 
==Transgenic Cells==
 
==Transgenic Cells==
 +
 +
 +
</div>

Revision as of 15:38, 27 October 2012

<- Back to Protocols

Mammalian Culture Media

"Wild type" stock cells (non-transgenic)

Cell line Incomplete media Supplements for complete media (required)
U2OS (adherent) McCoy's 5A 10% FBS, 1 % pen/strep
K-562 (suspension) Iscove's Modified Dulbecco's Medium (IMDM) 10% FBS, 1 % pen/strep


  • FBS = fetal bovine serum
  • FBS-tf = tetracycline-free FBS (required to maintain the "off" state of dox-inducible transgenes)
  • pen/strep = penicillin and streptomycin anti fungal supplement
  • Filter sterilize all media before use
  • Store all media at 4°C

Transgenic Cells