Difference between revisions of "Haynes:MamCultureMedia"

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(New page: {| class="wikitable" |- | '''Cell line''' || '''Incomplete media''' || '''Supplements for complete media (required)''' || '''Transgene selection''' |- | U2OS (adherent) || McCoy's 5A || 10...)
 
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=Mammalian Culture Media=
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=="Wild type" stock cells (non-transgenic)==
 
{| class="wikitable"
 
{| class="wikitable"
 
|-
 
|-
| '''Cell line''' || '''Incomplete media''' || '''Supplements for complete media (required)''' || '''Transgene selection'''
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| '''Cell line''' || '''Incomplete media''' || '''Supplements for complete media (required)'''
 
|-
 
|-
| U2OS (adherent) || McCoy's 5A || 10% FBS, 1 % pen/strep || ---
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| U2OS (adherent) || McCoy's 5A || 10% FBS, 1 % pen/strep  
 
|-
 
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| K-562 (suspension) ||  
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| K-562 (suspension) || Iscove's Modified Dulbecco's Medium (IMDM)  || 10% FBS, 1 % pen/strep
 
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* Filter sterilize all media before use
 
* Filter sterilize all media before use
 
* Store all media at 4°C
 
* Store all media at 4°C
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==Transgenic Cells==

Revision as of 15:28, 27 October 2012

Mammalian Culture Media

"Wild type" stock cells (non-transgenic)

Cell line Incomplete media Supplements for complete media (required)
U2OS (adherent) McCoy's 5A 10% FBS, 1 % pen/strep
K-562 (suspension) Iscove's Modified Dulbecco's Medium (IMDM) 10% FBS, 1 % pen/strep


  • FBS = fetal bovine serum
  • FBS-tf = tetracycline-free FBS (required to maintain the "off" state of dox-inducible transgenes)
  • pen/strep = penicillin and streptomycin anti fungal supplement
  • Filter sterilize all media before use
  • Store all media at 4°C

Transgenic Cells