Griffitts:Stock solutions: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
m (→10 mM dNTPs) |
|||
| Line 45: | Line 45: | ||
==DNA== | ==DNA== | ||
===10 mM dNTPs=== | ===10 mM dNTPs=== | ||
* Thaw and mix 100 mM stock solutions of G, A, T, C (NEB) | * Thaw and mix (stir) 100 mM stock solutions of G, A, T, C (NEB) | ||
* Combine in a 1.5-mL tube: | * Combine in a 1.5-mL tube: | ||
** 60 μl H<sub>2</sub>O | ** 60 μl H<sub>2</sub>O | ||
Revision as of 10:10, 17 January 2008
Antibiotics
Ampicillin (40 mL)
- 1 g Ampicillin
- 40 mL ddH2O
- Filter sterilize
- Store in refrigerator
Chloramphenicol (40 mL)
- 0.3 g Chloramphenicol
- 40 mL Ethanol
- Do not filter sterilize
- Store in refrigerator
Gentamycin (40 mL)
- 0.3 g Gentamycin
- 40 mL ddH2O
- Filter sterilize
- Store in refrigerator
Hygromycin (40 mL)
Kanamycin (40 mL)
- 0.1 g Kanamycin
- 40 mL ddH2O
- Filter sterilize
- Store in refrigerator
Neomycin (40 mL)
- 0.8 g Neomycin
- 40 mL ddH2O
- Filter sterilize
- Store in refrigerator
Spectinomycin (40 mL)
- 20 mg Spectinomycin
- 40 mL ddH2O
- Filter sterilize
- Store in refrigerator
Streptomycin (40 mL)
- 2.0 g Streptomycin
- 40 mL ddH2O
- Filter sterilize
- Store in refrigerator
Tetracycline (40 mL)
- 0.1 g Tetracycline
- 40 mL Ethanol
- Do not filter sterilize
- Store in refrigerator
DNA
10 mM dNTPs
- Thaw and mix (stir) 100 mM stock solutions of G, A, T, C (NEB)
- Combine in a 1.5-mL tube:
10X Taq buffer
10X Pfx buffer
20X Triton X-100
Media Additives
1 M Calcium Chloride (75 mL)
- 75 mL dH2O
- 11.027 g CaCl2·2H2O
Autoclave
