Difference between revisions of "Griffitts:Protocols"

From OpenWetWare
Jump to: navigation, search
(Bacteria)
m (Bacteria)
Line 34: Line 34:
 
* [[Griffitts:Culture tips|Tips on streaking/culturing]]
 
* [[Griffitts:Culture tips|Tips on streaking/culturing]]
 
* [[Griffitts:Freezing bacteria|Freezing procedure]]
 
* [[Griffitts:Freezing bacteria|Freezing procedure]]
* [[Griffitts:Bacterial DNA prep|Genomic DNA prep]]
+
* [[Griffitts:Bacterial DNA preparation|Genomic DNA prep]]
* [[Griffitts:Bacterial RNA prep|RNA prep (Trizol)]]
+
* [[Griffitts:Bacterial RNA preparation|RNA prep (Trizol)]]
 
* [[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]]
 
* [[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]]
 
* [[Griffitts:Triparental Mating|Triparental Mating]]
 
* [[Griffitts:Triparental Mating|Triparental Mating]]

Revision as of 12:06, 6 November 2007

Grifbanner2.jpg


Recipes

DNA

RNA

  • Trizol purification (bacterial)
  • DNAse treatment
  • Reverse transcription
  • 5'RACE

Protein

  • Gel recipes
  • Sample buffer
  • Bacterial lysis
  • 6His purification (6His)
  • MBP purification (MBP)

Bacteria

Plants