Difference between revisions of "Griffitts:Protocols"

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(DNA)
m (Bacteria)
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==Bacteria==
 
==Bacteria==
*[[Griffitts:Common media#Bacterial Media and Additives|Media]]
+
* [[Griffitts:Common media#Bacterial Media and Additives|Media]]
*Antibiotics
+
* [[Griffitss:Stock_solutions#Antibiotics|Antibiotics]]
*Tips on streaking/culturing
+
* Tips on streaking/culturing
*Freezing Procedure
+
* Freezing Procedure
*Genomic DNA prep
+
* Genomic DNA prep
*RNA prep (Trizol)
+
* RNA prep (Trizol)
*[[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]]
+
* [[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]]
*Triparental Mating
+
* Triparental Mating
*Transduction
+
* Transduction
  
 
==Plants==
 
==Plants==

Revision as of 11:37, 6 November 2007

Grifbanner2.jpg


Recipes

DNA

RNA

  • Trizol purification (bacterial)
  • DNAse treatment
  • Reverse transcription
  • 5'RACE

Protein

  • Gel recipes
  • Sample buffer
  • Bacterial lysis
  • 6His purification (6His)
  • MBP purification (MBP)

Bacteria

Plants