Difference between revisions of "Griffitts:Protocols"

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(DNA)
m (Plants)
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* [[Griffitts:Seed scarification and germination|Seed scarification and germination]]
 
* [[Griffitts:Seed scarification and germination|Seed scarification and germination]]
 
* [[Griffitts:Plant tips|Tips for growth/seed storage]]
 
* [[Griffitts:Plant tips|Tips for growth/seed storage]]
* [[Griffitts:DNA preparation|DNA prep]]
+
* [[Griffitts:Plant DNA preparation|DNA prep]]
* [[Griffitts:RNA preparation|RNA prep]]
+
* [[Griffitts:Plant RNA preparation|RNA prep]]
 
* [[Griffitts:Seed Storage|Seed Storage]]
 
* [[Griffitts:Seed Storage|Seed Storage]]
 
* [[Griffitts:Microscopy|Microscopy]]
 
* [[Griffitts:Microscopy|Microscopy]]

Revision as of 15:38, 1 November 2007

Grifbanner2.jpg


Recipes

DNA

RNA

  • Trizol purification (bacterial)
  • DNAse treatment
  • Reverse transcription
  • 5'RACE

Protein

  • Gel recipes
  • Sample buffer
  • Bacterial lysis
  • 6His purification (6His)
  • MBP purification (MBP)

Bacteria

  • Media
  • Antibiotics
  • Tips on streaking/culturing
  • Freezing Procedure
  • Genomic DNA prep
  • RNA prep (Trizol)
  • ß-Gal/GUS assay
  • Triparental Mating
  • Transduction

Plants