Difference between revisions of "Griffitts:Protocols"

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m (Plants)
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==Plants==
 
==Plants==
*[[Griffitts:Common media#Plant Media and Additives|Media]]
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* [[Griffitts:Common media#Plant Media and Additives|Media]]
*Seed scarification
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* [[Griffitts:Seed scarification|Seed scarification]]
*Tips for growth/seed storage
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* [[Griffitts:Plant tips|Tips for growth/seed storage]]
*DNA prep
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* [[Griffitts:DNA preparation|DNA prep]]
*RNA prep
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* [[Griffitts:RNA preparation|RNA prep]]
*Seed Storage
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* [[Griffitts:Seed Storage|Seed Storage]]
*Microscopy
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* [[Griffitts:Microscopy|Microscopy]]

Revision as of 06:43, 12 October 2007

Grifbanner2.jpg


Recipes

DNA

RNA

  • Trizol purification (bacterial)
  • DNAse treatment
  • Reverse transcription
  • 5'RACE

Protein

  • Gel recipes
  • Sample buffer
  • Bacterial lysis
  • 6His purification (6His)
  • MBP purification (MBP)

Bacteria

  • Media
  • Antibiotics
  • Tips on streaking/culturing
  • Freezing Procedure
  • Genomic DNA prep
  • RNA prep (Trizol)
  • ß-Gal/GUS assay
  • Triparental Mating
  • Transduction

Plants