Griffitts:Protocols: Difference between revisions
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*[[Griffitts:Miniprep|Qiagen Miniprep]] | *[[Griffitts:Miniprep|Qiagen Miniprep]] | ||
*Low-copy plasmid prep | *Low-copy plasmid prep | ||
*[[Griffitts:TaqPCR| | *[[Griffitts:TaqPCR|Standard PCR]] | ||
*Pfx PCR | *Pfx PCR | ||
*Overlap-extension PCR | *Overlap-extension PCR | ||
Revision as of 11:36, 2 October 2007
Recipes
DNA
- Qiagen Miniprep
- Low-copy plasmid prep
- Standard PCR
- Pfx PCR
- Overlap-extension PCR
- Arbitrary PCR
- Restriction digest
- In-gel ligation
RNA
- Trizol purification (bacterial)
- DNAse treatment
- Reverse transcription
- 5'RACE
Protein
- Gel recipes
- Sample buffer
- Bacterial lysis
- 6His purification (6His)
- MBP purification (MBP)
Bacteria
- Media
- Antibiotics
- Tips on streaking/culturing
- Freezing Procedure
- Genomic DNA prep
- RNA prep (Trizol)
- ß-Gal/GUS assay
- Triparental Mating
- Transduction
Plants
- Media
- Seed scarification
- Tips for growth/seed storage
- DNA prep
- RNA prep
- Seed Storage
- Microscopy
