Difference between revisions of "Griffitts:Protocols"

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(Plants)
(Protein)
Line 19: Line 19:
 
*5'RACE
 
*5'RACE
 
==Protein==
 
==Protein==
*Gel recipes
+
*[[Gel recipes]]
 
*Sample buffer
 
*Sample buffer
 
*Bacterial lysis
 
*Bacterial lysis
 
*6His purification (6His)
 
*6His purification (6His)
 
*MBP purification (MBP)
 
*MBP purification (MBP)
 +
 
==Bacteria==
 
==Bacteria==
 
*[[Griffitts:Common media#Bacterial Media and Additives|Media]]
 
*[[Griffitts:Common media#Bacterial Media and Additives|Media]]

Revision as of 11:11, 8 September 2007

Grifbanner2.jpg


Recipes

DNA

  • Qiagen Miniprep
  • Low-copy plasmid prep
  • Taq PCR
  • Pfx PCR
  • Overlap-extension PCR
  • Arbitrary PCR
  • Restriction digest
  • In-gel ligation

RNA

  • Trizol purification (bacterial)
  • DNAse treatment
  • Reverse transcription
  • 5'RACE

Protein

  • Gel recipes
  • Sample buffer
  • Bacterial lysis
  • 6His purification (6His)
  • MBP purification (MBP)

Bacteria

  • Media
  • Antibiotics
  • Tips on streaking/culturing
  • Freezing Procedure
  • Genomic DNA prep
  • RNA prep (Trizol)
  • Beta-gal/gus assays
  • Triparental Mating
  • Transduction

Plants

  • Media
  • Seed scarification
  • Tips for growth/seed storage
  • DNA prep
  • RNA prep
  • Seed Storage
  • Microscopy