Difference between revisions of "Glutaraldehyde (Pentanedial)"

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== Properties ==
 
== Properties ==
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* Glutaraldehyde is a potent fixative, and should only be used in a chemical fume hood. Do not dispose of excess fix or glutaraldehyde solution in sink
  
 
== Uses ==
 
== Uses ==
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* [[Bitan:Electron_microscopy|EM protocol]] by the [[Bitan|Bitan lab]] using glutaraldehyde as fixative
 
* [[Bitan:Electron_microscopy|EM protocol]] by the [[Bitan|Bitan lab]] using glutaraldehyde as fixative
 
* [[X-gal_Staining_Protocol|Mixed 2% PFA, 0.2% GA for X-gal stainings]]
 
* [[X-gal_Staining_Protocol|Mixed 2% PFA, 0.2% GA for X-gal stainings]]
* [[Oneill_Lab:WMISH_Chemicals|Recipes for a mixed fixative containing 0.1% glutaraldehyde]] from the [[Oneill_Lab||O'Neill lab]]
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* [[Oneill_Lab:WMISH_Chemicals|Recipes for a mixed fixative containing 0.1% glutaraldehyde]] from the [[Oneill_Lab|O'Neill lab]]
 
* [[DIYbio/FAQ/Methods|Glutaraldehyde as disinfectant]]
 
* [[DIYbio/FAQ/Methods|Glutaraldehyde as disinfectant]]
  

Revision as of 05:13, 3 November 2011

Chemical structure of glutaraldehyde (1,5-pentane-dial)

Glutaraldehyde (1,5-pentanedial) is a common fixative in biology. It is used to reduce degradation in cells, tissues, and entire organisms before further experiments like electron microscopy.

Properties

  • Glutaraldehyde is a potent fixative, and should only be used in a chemical fume hood. Do not dispose of excess fix or glutaraldehyde solution in sink

Uses

  • Glutaraldehyde is used to fix specimen before electron microscopy where it is employed alone or mixed with polymethanal (paraformaldehyde) as the first of 2 fixations followed by osmium tetroxide.
  • Glutaraldehyde is used as an amine cross-linker.
  • Glutaraldehyde is used in SDS-PAGE to fix/crosslink proteins and peptides prior to staining. Gels are treated with a 5% solution for ~30 min, after which it must be thoroughly washed to remove the yellow stain brought about by reacting with free Tris. Alternatively, gels can be washed before fixation.

Recipes

Links