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Revision as of 11:51, 23 April 2013 by Jacqueline K. Grimm (talk | contribs) (New page: 1M Tris-HCl, pH 7.5 ==Materials== 1000 mL: *Tris base 121.12 g *dH<sub>2</sub>O 800 mL *HCl ==Procedure== Dissolve in 800 ml dH<sub>2</sub>O, adjust pH to 7.5 with HCl, add water to...)
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1M Tris-HCl, pH 7.5


1000 mL:

  • Tris base 121.12 g
  • dH2O 800 mL
  • HCl


Dissolve in 800 ml dH2O, adjust pH to 7.5 with HCl, add water to a final volume of 1000 ml. Aliquot as appropriate, autoclave, store at room temp.


Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!