Gill:Tris-HCl

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Revision as of 11:51, 23 April 2013 by Jacqueline K. Grimm (talk | contribs) (New page: 1M Tris-HCl, pH 7.5 ==Materials== 1000 mL: *Tris base 121.12 g *dH<sub>2</sub>O 800 mL *HCl ==Procedure== Dissolve in 800 ml dH<sub>2</sub>O, adjust pH to 7.5 with HCl, add water to...)
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1M Tris-HCl, pH 7.5

Materials

1000 mL:

  • Tris base 121.12 g
  • dH2O 800 mL
  • HCl

Procedure

Dissolve in 800 ml dH2O, adjust pH to 7.5 with HCl, add water to a final volume of 1000 ml. Aliquot as appropriate, autoclave, store at room temp.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!