Difference between revisions of "Gill:Tris-HCl"

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==Notes==
 
==Notes==
 
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
 
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
 +
 +
*If you overshoot the pH, don't add another base. You can add more Tris, but it will change the final concentration.
  
 
[[Category:Protocol]]
 
[[Category:Protocol]]

Latest revision as of 11:44, 3 June 2013

1M Tris-HCl, pH 7.5

Materials

1000 mL:

  • 121.12 g Tris base
  • 800 mL dH2O
  • HCl

Procedure

Dissolve in 800 ml dH2O, adjust pH to 7.5 with HCl, add water to a final volume of 1000 ml. Aliquot as appropriate, autoclave, store at room temp.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  • If you overshoot the pH, don't add another base. You can add more Tris, but it will change the final concentration.