Gill:TSB (Tryptone Soy Broth)

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Revision as of 12:57, 23 April 2013 by Jacqueline K. Grimm (talk | contribs) (New page: ==Materials== 500 mL: *TSB (Bacto) 15 g *dH<sub>2</sub>O 500 ml 1000 mL: *TSB (Bacto) 30 g *dH<sub>2</sub>O 1000 ml ==Procedure== Dissolve medium in water, then aliquot 100 ml i...)
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Materials

500 mL:

  • TSB (Bacto) 15 g
  • dH2O 500 ml

1000 mL:

  • TSB (Bacto) 30 g
  • dH2O 1000 ml

Procedure

Dissolve medium in water, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at 4 ºC in the cold room.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!